pAD-LyseR
(Plasmid #99244)

• Purpose: For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
• Depositing Lab: Jeff Hasty
• Publication: Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.1021/acssynbio.7b00253.

Backbone

• Vector backbone: pPro24
• Backbone manufacturer: Jay Keasling lab
• Total vector size (bp): 3790
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21-Gold (DE3)
• Growth instructions: Use 24% glycerol (w/v) in glycerol stocks to avoid cell lysis upon a freeze-thaw cycle.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phage lambda gene R
• Alt name: phage lambda endolysin
• Species: phage lambda
• Insert Size (bp): 474
• Entrez Gene: R (a.k.a. lambdap75)
• Promoter: beta-lactamase promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCACATAGCAGAACTTTAAAAGTGCT
• 3′ sequencing primer: CCG GGA GCT GCA TGT GTC AGA GG

Resource Information

• Supplemental Documents:
  • pAD-LyseR.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pAD-LyseR plasmid in BL21-Gold (DE3) strain is used for producing autolysate for in vitro transcription-translation reactions.