pDB070.iodo.5
(Plasmid #99397)

• Purpose: Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine
• Depositing Lab: David Liu
• Publication: Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474.

Backbone

• Vector backbone: p15A
• Backbone manufacturer: David Liu Lab
• Backbone size w/o insert (bp): 1921
• Total vector size (bp): 4682
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
• Species: Synthetic
• Insert Size (bp): 2761
• Promoter: PN25, Ptet, and PproK

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pDB070.iodo5 (without duplication).gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that a portion of the plasmid is duplicated. This duplication should not affect the overall plasmid function. If needed, the duplication can be removed by digesting the plasmid with NheI and ligating the the larger (4.7 kb) fragment. Please see the documents under "Resource Information" for reference.