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Pathway engineered enzymatic de novo purine nucleotide synthesis.

Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73623pET22-HT/purCProduces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag
73624pET22-HT/prsAProduces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag
73625pET22-HT/plsAProduces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag
73631pET22-HT/spoRProduces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Antibodies from Article