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(Plasmid #73631)


Item Catalog # Description Quantity Price (USD)
Plasmid 73631 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    This form of pET22b-HT adds a 19-residue sequence including a N-terminal His6 tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    guanylate kinase
  • Alt name
  • Alt name
  • Alt name
    b3648, JW3623
  • Species
    Escherichia coli
  • GenBank ID
    AAC76672.1 WP_001295237.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Source was E. coli K-12 MRE6000

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22-HT/spoR was a gift from T. J. Kappock & James Williamson (Addgene plasmid # 73631 ; ; RRID:Addgene_73631)
  • For your References section:

    Pathway engineered enzymatic de novo purine nucleotide synthesis. Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR. ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p. 10.1021/cb800066p PubMed 18707057