pET22-HT/spoR
(Plasmid #73631)

• Purpose: Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag
• Depositing Labs: T. J. Kappock, James Williamson
• Publication: Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.

Backbone

• Vector backbone: pET22b
• Modifications to backbone: This form of pET22b-HT adds a 19-residue sequence including a N-terminal His6 tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: guanylate kinase
• Alt name: gmk
• Alt name: spoR
• Alt name: b3648, JW3623
• Species: Escherichia coli
• GenBank ID: AAC76672.1 WP_001295237.1
• Promoter: T7
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Source was E. coli K-12 MRE6000