Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9.
Paquet D, Kwart D, Chen A, Sproul A, Jacob S, Teo S, Olsen KM, Gregg A, Noggle S, Tessier-Lavigne M
Nature. 2016 May 5;533(7601):125-9. doi: 10.1038/nature17664. Epub 2016 Apr 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75475 | pCas9-VRER_2A_GFP | Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control |