Generating permissive site-specific unnatural aminoacyl-tRNA synthetases.
Miyake-Stoner SJ, Refakis CA, Hammill JT, Lusic H, Hazen JL, Deiters A, Mehl RA
Biochemistry. 2010 Mar 2;49(8):1667-77. doi: 10.1021/bi901947r.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85482 | pBad-sfGFP | Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag |
| 85483 | pBad-sfGFP 150TAG | Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150 |
| 85484 | pDule-tfmF A65V S158A | Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA |