Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.
Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K
Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051. PubMed Article

Plasmids from Article

ID Plasmid Purpose
80766pDonor-tBFP-NLS-NeoCustomizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.
80767pDonor-tBFP-NLS-Neo (Universal)Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.
80768peSpCas9(1.1)-2×sgRNA (empty, donor)All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.
80769peSpCas9(1.1)-2×sgRNA (IFT88, donor)Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

Antibodies from Article