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CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
83889pLV-dCas9-p300-P2A-PuroRLentiviral Sp dCas9-p300-P2A-PuroR
83890pLV-dCas9-KRAB-PGK-HygRLentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
83919pLV-U6-gRNA-UbC-DsRed-P2A-BsrLentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR
83925pLV-U6-gRNA-UbC-eGFP-P2A-BsrLentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Antibodies from Article