CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome.
Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. PubMed Article
Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA
Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83889 | pLV-dCas9-p300-P2A-PuroR | Lentiviral Sp dCas9-p300-P2A-PuroR |
| 83890 | pLV-dCas9-KRAB-PGK-HygR | Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette. |
| 83919 | pLV-U6-gRNA-UbC-DsRed-P2A-Bsr | Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR |
| 83925 | pLV-U6-gRNA-UbC-eGFP-P2A-Bsr | Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR |