Using CRISPR-Cas9-mediated genome editing to generate C. difficile mutants defective in selenoproteins synthesis.

McAllister KN, Bouillaut L, Kahn JN, Self WT, Sorg JA
Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.1038/s41598-017-15236-5. PubMed

Plasmids from Article

ID	Plasmid	Purpose
105133	pJK02	Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT
105134	pKM126	Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT