Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA).
Chauhan N, Hatlem D, Orwick-Rydmark M, Schneider K, Floetenmeyer M, van Rossum B, Leo JC, Linke D
Mol Microbiol. 2019 Jan 1. doi: 10.1111/mmi.14195.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107420 | pIBA3-SpyCatcher-sfGFP | For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection. |
| 107421 | pIBA3-SpyCatcherEQ-sfGFP | For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection. |
| 107422 | pIBA2-SpyTag-MBP | Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting. |