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(Plasmid #107421)


Item Catalog # Description Quantity Price (USD)
Plasmid 107421 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    IBA GmbH
  • Backbone size w/o insert (bp) 3226
  • Total vector size (bp) 4234
  • Modifications to backbone
    deletion of residues 142-216
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    mutation of glutamate 77 to glutamine
  • GenBank ID
  • Promoter Tet
  • Tags / Fusion Proteins
    • His tag (N terminal on insert)
    • superfolder GFP (C terminal on insert)
    • StrepII (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAGTTATTTTACCACTCCCT
  • 3′ sequencing primer CGCAGTAGCGGTAAACG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene (Mark Howarth lab)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7; amino acid numbering is based on the sequence in this article.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIBA3-SpyCatcherEQ-sfGFP was a gift from Jack Leo (Addgene plasmid # 107421 ; ; RRID:Addgene_107421)
  • For your References section:

    Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA). Chauhan N, Hatlem D, Orwick-Rydmark M, Schneider K, Floetenmeyer M, van Rossum B, Leo JC, Linke D. Mol Microbiol. 2019 Jan 1. doi: 10.1111/mmi.14195. 10.1111/mmi.14195 PubMed 30600549