PurposeFor production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107421||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerIBA GmbH
- Backbone size w/o insert (bp) 3226
- Total vector size (bp) 4234
Modifications to backbonedeletion of residues 142-216
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Insert Size (bp)1083
Mutationmutation of glutamate 77 to glutamine
- Promoter Tet
/ Fusion Proteins
- His tag (N terminal on insert)
- superfolder GFP (C terminal on insert)
- StrepII (C terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer GAGTTATTTTACCACTCCCT
- 3′ sequencing primer CGCAGTAGCGGTAAACG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7; amino acid numbering is based on the sequence in this article.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIBA3-SpyCatcherEQ-sfGFP was a gift from Jack Leo (Addgene plasmid # 107421 ; http://n2t.net/addgene:107421 ; RRID:Addgene_107421)
For your References section:Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA). Chauhan N, Hatlem D, Orwick-Rydmark M, Schneider K, Floetenmeyer M, van Rossum B, Leo JC, Linke D. Mol Microbiol. 2019 Jan 1. doi: 10.1111/mmi.14195. 10.1111/mmi.14195 PubMed 30600549