Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #52656)


Item Catalog # Description Quantity Price (USD)
Plasmid 52656 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6532
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    TEM-1 β-Lactamase
  • Alt name
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
  • GenBank ID
    YP_006952162.1 KJ645919
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • SpyTag (N terminal on insert)
    • SpyCatcher (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpyTag-β-Lactamase-SpyCatcher was a gift from Mark Howarth (Addgene plasmid # 52656 ; ; RRID:Addgene_52656)
  • For your References section:

    SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme. Schoene C, Fierer JO, Bennett SP, Howarth M. Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. doi: 10.1002/anie.201402519. Epub 2014 May 9. 10.1002/anie.201402519 PubMed 24817566