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SpyTag-β-Lactamase-SpyCatcher
(Plasmid #52656)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52656 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6532
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEM-1 β-Lactamase
  • Alt name
    BLA
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
    1284
  • GenBank ID
    YP_006952162.1 KJ645919
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • SpyTag (N terminal on insert)
    • SpyCatcher (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpyTag-β-Lactamase-SpyCatcher was a gift from Mark Howarth (Addgene plasmid # 52656)
  • For your References section:

    SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme. Schoene C, Fierer JO, Bennett SP, Howarth M. Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. doi: 10.1002/anie.201402519. Epub 2014 May 9. 10.1002/anie.201402519 PubMed 24817566