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pDEST14-SpyCatcher
(Plasmid #35044)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35044 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST14
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher
  • Alt name
    CnaB2114
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    420
  • Mutation
    Deletion of C-terminal till I113, and point mutations I34E and M69Y
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST14-SpyCatcher was a gift from Mark Howarth (Addgene plasmid # 35044 ; http://n2t.net/addgene:35044 ; RRID:Addgene_35044)
  • For your References section:

    Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. 10.1073/pnas.1115485109 PubMed 22366317