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pDEST14-SpyCatcher Citations (13)

Originally described in: Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24.
PubMed Journal

Articles Citing pDEST14-SpyCatcher

A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler. Leonard JD, Narlikar GJ. Mol Cell. 2015 Mar 5;57(5):850-859. doi: 10.1016/j.molcel.2015.01.008. Epub 2015 Feb 12. PubMed
Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers. Botyanszki Z, Tay PK, Nguyen PQ, Nussbaumer MG, Joshi NS. Biotechnol Bioeng. 2015 May 6. doi: 10.1002/bit.25638. PubMed
Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. PubMed

Associated Plasmids

A new method for post-translationally labeling proteins in live cells for fluorescence imaging and tracking. Hinrichsen M, Lenz M, Edwards JM, Miller OK, Mochrie SGJ, Swain PS, Schwarz-Linek U, Regan L. Protein Eng Des Sel. 2017 Dec 1;30(12):771-780. doi: 10.1093/protein/gzx059. PubMed
SpyTag/SpyCatcher Functionalization of E2 Nanocages with Stimuli-Responsive Z-ELP Affinity Domains for Tunable Monoclonal Antibody Binding and Precipitation Properties. Swartz AR, Chen W. Bioconjug Chem. 2018 Sep 19;29(9):3113-3120. doi: 10.1021/acs.bioconjchem.8b00458. Epub 2018 Aug 22. PubMed
Insights into the autotransport process of a trimeric autotransporter, Yersinia Adhesin A (YadA). Chauhan N, Hatlem D, Orwick-Rydmark M, Schneider K, Floetenmeyer M, van Rossum B, Leo JC, Linke D. Mol Microbiol. 2019 Jan 1. doi: 10.1111/mmi.14195. PubMed

Associated Plasmids

Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Methods Enzymol. 2019;617:443-461. doi: 10.1016/bs.mie.2018.12.010. Epub 2019 Jan 25. PubMed
Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning. Donovan DA, Crandall JG, Banks OGB, Jensvold ZD, Truong V, Dinwiddie D, McKnight LE, McKnight JN. Cell Rep. 2019 Nov 19;29(8):2520-2535.e4. doi: 10.1016/j.celrep.2019.10.046. PubMed
A generic cell surface ligand system for studying cell-cell recognition. Denham EM, Barton MI, Black SM, Bridge MJ, de Wet B, Paterson RL, van der Merwe PA, Goyette J. PLoS Biol. 2019 Dec 9;17(12):e3000549. doi: 10.1371/journal.pbio.3000549. eCollection 2019 Dec. PubMed
Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity. Izore T, Candace Ho YT, Kaczmarski JA, Gavriilidou A, Chow KH, Steer DL, Goode RJA, Schittenhelm RB, Tailhades J, Tosin M, Challis GL, Krenske EH, Ziemert N, Jackson CJ, Cryle MJ. Nat Commun. 2021 May 4;12(1):2511. doi: 10.1038/s41467-021-22623-0. PubMed
DogCatcher allows loop-friendly protein-protein ligation. Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 10.1016/j.chembiol.2021.07.005. PubMed

Associated Plasmids

N-Terminal Modification of Gly-His-Tagged Proteins with Azidogluconolactone. Brune KD, Lieknina I, Sutov G, Morris AR, Jovicevic D, Kalnins G, Kazaks A, Kluga R, Kastaljana S, Zajakina A, Jansons J, Skrastina D, Spunde K, Cohen AA, Bjorkman PJ, Morris HR, Suna E, Tars K. Chembiochem. 2021 Nov 16;22(22):3199-3207. doi: 10.1002/cbic.202100381. Epub 2021 Oct 6. PubMed
Quaternary structure independent folding of voltage-gated ion channel pore domain subunits. Arrigoni C, Lolicato M, Shaya D, Rohaim A, Findeisen F, Fong LK, Colleran CM, Dominik P, Kim SS, Schuermann JP, DeGrado WF, Grabe M, Kossiakoff AA, Minor DL Jr. Nat Struct Mol Biol. 2022 Jun;29(6):537-548. doi: 10.1038/s41594-022-00775-x. Epub 2022 Jun 2. PubMed

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