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Programmable polyproteams built using twin peptide superglues.
Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M
Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
72322pET28a-SnoopCatcherBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
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72323pET28a SnoopTag-MBPBacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
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72324pET28a SpyCatcher-SnoopCatcherBacterial expression of SpyCatcher linked to SnoopCatcher.
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72325pET28a SnoopTag-mEGFP-SpyTagBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
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72326AviTag-SpyCatcherBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.
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72327pET21 MBPx-SpyCatcherBacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
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