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pET21 MBPx-SpyCatcher
(Plasmid #72327)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72327 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5442
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pET21 MBPx-SpyCatcher
  • Alt name
    MBPx-SpyCatcher
  • Species
    Synthetic
  • Insert Size (bp)
    2661
  • Mutation
    MBP contains A312V, I317V mutations and 172, 173, 175 and 176 deletions
  • Tag / Fusion Protein
    • N-terminal His6 tag

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer GCT AGT TAT TGC TCA GCG G
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21 MBPx-SpyCatcher was a gift from Mark Howarth (Addgene plasmid # 72327)
  • For your References section:

    Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. 10.1073/pnas.1519214113 PubMed 26787909