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AviTag-SpyCatcher
(Plasmid #72326)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72326 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST14-SpyCatcher
  • Backbone manufacturer
    Howarth lab (Addgene plasmid #35044)
  • Backbone size w/o insert (bp) 6422
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AviTag-SpyCatcher
  • Species
    Synthetic
  • Insert Size (bp)
    474
  • Tag / Fusion Protein
    • N-term His6 tag and TEV cleavage site

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AviTag-SpyCatcher was a gift from Mark Howarth (Addgene plasmid # 72326)
  • For your References section:

    Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. 10.1073/pnas.1519214113 PubMed 26787909