|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72324||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5442
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namepET28a SpyCatcher-SnoopCatcher
Insert Size (bp)774
Mutation35 amino acids at the N-term of SpyCatcher have been deleted
/ Fusion Protein
- N-terminal His6 tag
- Cloning method Unknown
- 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG (Common Sequencing Primers)
Terms and Licenses
Please note that the bacterial resistance of this plasmid is ampicillin, not kanamycin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a SpyCatcher-SnoopCatcher was a gift from Mark Howarth (Addgene plasmid # 72324)
For your References section:Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. 10.1073/pnas.1519214113 PubMed 26787909