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Addgene

pET28a SnoopTag-MBP
(Plasmid #72323)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72323 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pET28a SnoopTag-MBP
  • Alt name
    SnoopTag-MBP
  • Species
    Synthetic
  • Insert Size (bp)
    1233
  • Tag / Fusion Protein
    • N-terminal His6 tag. MBP is fused at the C-terminus of SnoopTag

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer GCT AGT TAT TGC TCA GCG G
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a SnoopTag-MBP was a gift from Mark Howarth (Addgene plasmid # 72323 ; http://n2t.net/addgene:72323 ; RRID:Addgene_72323)
  • For your References section:

    Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. 10.1073/pnas.1519214113 PubMed 26787909