PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||171775||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6401
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)1032
MutationDogTag inserted in HaloTag7 between residues D139 and E140 and C61S and C261S mutations in HaloTag7 to block disulfide bond formation
- Promoter T7
/ Fusion Protein
- His6 (N terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a-HaloTag7SS-DogTag was a gift from Mark Howarth (Addgene plasmid # 171775 ; http://n2t.net/addgene:171775 ; RRID:Addgene_171775)
For your References section:DogCatcher allows loop-friendly protein-protein ligation. Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. Cell Chem Biol. 2021 Jul 28. pii: S2451-9456(21)00315-9. doi: 10.1016/j.chembiol.2021.07.005. 10.1016/j.chembiol.2021.07.005 PubMed 34324879