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RhoA Controls Axon Extension Independent of Specification in the Developing Brain.

Dupraz S, Hilton BJ, Husch A, Santos TE, Coles CH, Stern S, Brakebusch C, Bradke F
Curr Biol. 2019 Nov 18;29(22):3874-3886.e9. doi: 10.1016/j.cub.2019.09.040. Epub 2019 Oct 31. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133922pCAG-loxSTOPlox-LynEGFPReporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination
133923pCAG-roxSTOProx-LynEGFPReporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Dre-based recombination
133924pTalpha1-iCreNeuron-specific expression of iCre
133925pTalpha1-DreNeuron-specific expression of Dre
133926pCMVd1-EGFP-hRhoA-wtLow level expression of GFP fused human RhoA wild type
133927pCMV-EB3mCherry_cmv-LifeActGFPLive dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton
133928pCAG-MLC2(CA)-IRES-GFPBicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain
133929pTalpha1-EGFP-N1Neuron-specific expression of EGFP

Antibodies from Article