pCMVd1-EGFP-hRhoA-wt
(Plasmid #133926)

• Purpose: Low level expression of GFP fused human RhoA wild type
• Depositing Labs: Frank Bradke, Sebastián Dupraz
• Publication: Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.e9. doi: 10.1016/j.cub.2019.09.040. Epub 2019 Oct 31.

Backbone

• Vector backbone: NA
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-hRhoA WT
• Species: H. sapiens (human)
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pFN22K-mCMVd1-EGFP-RhoA(WT).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry