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tetO-FUW-eGFP-RHOA-Q63L
(Plasmid #73081)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73081 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RHOA-Q63L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    582
  • Mutation
    Q63L Constitutively active
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, RHO12, RHOH12)
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GTGCCCACAGTGTTTGAGAA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tetO-FUW-eGFP-RHOA-Q63L was a gift from Andrew Putnam (Addgene plasmid # 73081 ; http://n2t.net/addgene:73081 ; RRID:Addgene_73081)
  • For your References section:

    Matrix identity and tractional forces influence indirect cardiac reprogramming. Kong YP, Carrion B, Singh RK, Putnam AJ. Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474. 10.1038/srep03474 PubMed 24326998