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PurposeLentiviral transfer vector for tet-inducible expression of EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 73083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneFUW
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Backbone manufacturerhomemade
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnhanced Green Fluorescent Protein
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Alt nameeGFP
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Insert Size (bp)714
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GCCGAGGTGAAGTTCGAG (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byTetO-FUW-OSKM (#20321). This vector came from the Whitehead Institute for Biomedical Research via Addgene.
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tetO-FUW-eGFP was a gift from Andrew Putnam (Addgene plasmid # 73083 ; http://n2t.net/addgene:73083 ; RRID:Addgene_73083) -
For your References section:
Matrix identity and tractional forces influence indirect cardiac reprogramming. Kong YP, Carrion B, Singh RK, Putnam AJ. Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474. 10.1038/srep03474 PubMed 24326998