tetO-FUW-eGFP
(Plasmid #73083)

• Purpose: Lentiviral transfer vector for tet-inducible expression of EGFP
• Depositing Lab: Andrew Putnam
• Publication: Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474.

Backbone

• Vector backbone: FUW
  (Search Vector Database)
• Backbone manufacturer: homemade
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced Green Fluorescent Protein
• Alt name: eGFP
• Insert Size (bp): 714

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GCCGAGGTGAAGTTCGAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TetO-FUW-OSKM (#20321). This vector came from the Whitehead Institute for Biomedical Research via Addgene.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Institut Pasteur Label License for cPPT
• Industry Terms:
  • Not Available to Industry