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PurposeLentiviral plasmid expressing the reverse tetracycline transactivator
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneFUW (Lenti-Lox-Ubi)
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Backbone manufacturerhomemade
- Backbone size w/o insert (bp) 7231
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsTOP10, 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameM2rtTA
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Speciesbacteria
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Insert Size (bp)792
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUW-M2rtTA was a gift from Rudolf Jaenisch (Addgene plasmid # 20342 ; http://n2t.net/addgene:20342 ; RRID:Addgene_20342) -
For your References section:
A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. 10.1016/j.stem.2008.08.014 PubMed 18786421
