FUW-M2rtTA
(Plasmid #20342)

• Purpose: Lentiviral plasmid expressing the reverse tetracycline transactivator
• Depositing Lab: Rudolf Jaenisch
• Publication: Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53.

Backbone

• Vector backbone: FUW (Lenti-Lox-Ubi)
  (Search Vector Database)
• Backbone manufacturer: homemade
• Backbone size w/o insert (bp): 7231
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: TOP10, 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: M2rtTA
• Species: bacteria
• Insert Size (bp): 792

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 128 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Institut Pasteur Label License for cPPT
• Industry Terms:
  • Not Available to Industry