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pTet-O-Ngn2-puro
(Plasmid #52047)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52047 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Tet-O-FUW
  • Backbone size w/o insert (bp) 7745
  • Total vector size (bp) 9861
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ngn2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1071
  • GenBank ID
    NM_009718.2
  • Entrez Gene
    Neurog2 (a.k.a. Ato, Atoh4, Math, Math4A, bHLH, bHLHa8, ng, ngn-2, ngn2)
  • Promoter tet-on
  • Tag / Fusion Protein
    • T2A-and puromycin resistant cassette (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTet-O-Ngn2-puro was a gift from Marius Wernig (Addgene plasmid # 52047 ; http://n2t.net/addgene:52047 ; RRID:Addgene_52047)
  • For your References section:

    Rapid single-step induction of functional neurons from human pluripotent stem cells. Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, Sudhof TC. Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029. 10.1016/j.neuron.2013.05.029 PubMed 23764284