Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pTet-O-Ngn2-puro Citations (43)

Originally described in: Rapid single-step induction of functional neurons from human pluripotent stem cells.
Zhang Y, Pak C, Han Y, Ahlenius H, Zhang Z, Chanda S, Marro S, Patzke C, Acuna C, Covy J, Xu W, Yang N, Danko T, Chen L, Wernig M, Sudhof TC Neuron. 2013 Jun 5;78(5):785-98. doi: 10.1016/j.neuron.2013.05.029. PubMed Journal

Articles Citing pTet-O-Ngn2-puro

Articles
Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells. Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, Brennand KJ. Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 10.1016/j.ymeth.2015.11.019. PubMed

Associated Plasmids
Inhibition of STEP61 ameliorates deficits in mouse and hiPSC-based schizophrenia models. Xu J, Hartley BJ, Kurup P, Phillips A, Topol A, Xu M, Ononenyi C, Foscue E, Ho SM, Baguley TD, Carty N, Barros CS, Muller U, Gupta S, Gochman P, Rapoport J, Ellman JA, Pittenger C, Aronow B, Nairn AC, Nestor MW, Lombroso PJ, Brennand KJ. Mol Psychiatry. 2016 Oct 18. doi: 10.1038/mp.2016.163. PubMed
Highly Expandable Human iPS Cell-Derived Neural Progenitor Cells (NPC) and Neurons for Central Nervous System Disease Modeling and High-Throughput Screening. Cheng C, Fass DM, Folz-Donahue K, MacDonald ME, Haggarty SJ. Curr Protoc Hum Genet. 2017 Jan 11;92:21.8.1-21.8.21. doi: 10.1002/cphg.33. PubMed
Human AML-iPSCs Reacquire Leukemic Properties after Differentiation and Model Clonal Variation of Disease. Chao MP, Gentles AJ, Chatterjee S, Lan F, Reinisch A, Corces MR, Xavy S, Shen J, Haag D, Chanda S, Sinha R, Morganti RM, Nishimura T, Ameen M, Wu H, Wernig M, Wu JC, Majeti R. Cell Stem Cell. 2017 Mar 2;20(3):329-344.e7. doi: 10.1016/j.stem.2016.11.018. Epub 2017 Jan 12. PubMed
Dissecting the Causal Mechanism of X-Linked Dystonia-Parkinsonism by Integrating Genome and Transcriptome Assembly. Aneichyk T, Hendriks WT, Yadav R, Shin D, Gao D, Vaine CA, Collins RL, Domingo A, Currall B, Stortchevoi A, Multhaupt-Buell T, Penney EB, Cruz L, Dhakal J, Brand H, Hanscom C, Antolik C, Dy M, Ragavendran A, Underwood J, Cantsilieris S, Munson KM, Eichler EE, Acuna P, Go C, Jamora RDG, Rosales RL, Church DM, Williams SR, Garcia S, Klein C, Muller U, Wilhelmsen KC, Timmers HTM, Sapir Y, Wainger BJ, Henderson D, Ito N, Weisenfeld N, Jaffe D, Sharma N, Breakefield XO, Ozelius LJ, Bragg DC, Talkowski ME. Cell. 2018 Feb 22;172(5):897-909.e21. doi: 10.1016/j.cell.2018.02.011. PubMed
Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics. Ordureau A, Paulo JA, Zhang W, Ahfeldt T, Zhang J, Cohn EF, Hou Z, Heo JM, Rubin LL, Sidhu SS, Gygi SP, Harper JW. Mol Cell. 2018 Apr 19;70(2):211-227.e8. doi: 10.1016/j.molcel.2018.03.012. Epub 2018 Apr 12. PubMed
Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression. Srikanth P, Lagomarsino VN, Pearse RV 2nd, Liao M, Ghosh S, Nehme R, Seyfried N, Eggan K, Young-Pearse TL. Transl Psychiatry. 2018 Nov 8;8(1):245. doi: 10.1038/s41398-018-0281-9. PubMed
Lipidomic Analysis of alpha-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Fanning S, Haque A, Imberdis T, Baru V, Barrasa MI, Nuber S, Termine D, Ramalingam N, Ho GPH, Noble T, Sandoe J, Lou Y, Landgraf D, Freyzon Y, Newby G, Soldner F, Terry-Kantor E, Kim TE, Hofbauer HF, Becuwe M, Jaenisch R, Pincus D, Clish CB, Walther TC, Farese RV Jr, Srinivasan S, Welte MA, Kohlwein SD, Dettmer U, Lindquist S, Selkoe D. Mol Cell. 2019 Mar 7;73(5):1001-1014.e8. doi: 10.1016/j.molcel.2018.11.028. Epub 2018 Dec 4. PubMed
A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons. Sridharan B, Hubbs C, Llamosas N, Kilinc M, Singhera FU, Willems E, Piper DR, Scampavia L, Rumbaugh G, Spicer TP. Sci Rep. 2019 Jun 21;9(1):9000. doi: 10.1038/s41598-019-45265-1. PubMed
Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses. Patzke C, Brockmann MM, Dai J, Gan KJ, Grauel MK, Fenske P, Liu Y, Acuna C, Rosenmund C, Sudhof TC. Cell. 2019 Oct 3;179(2):498-513.e22. doi: 10.1016/j.cell.2019.09.011. PubMed
GSKIP-Mediated Anchoring Increases Phosphorylation of Tau by PKA but Not by GSK3beta via cAMP/PKA/GSKIP/GSK3/Tau Axis Signaling in Cerebrospinal Fluid and iPS Cells in Alzheimer Disease. Ko HJ, Chiou SJ, Wong YH, Wang YH, Lai Y, Chou CH, Wang C, Loh JK, Lieu AS, Cheng JT, Lin YT, Lu PJ, Fann MJ, Huang CF, Hong YR. J Clin Med. 2019 Oct 21;8(10). pii: jcm8101751. doi: 10.3390/jcm8101751. PubMed
Assessing the therapeutic potential of Graptopetalum paraguayense on Alzheimer's disease using patient iPSC-derived neurons. Wu PC, Fann MJ, Tran TT, Chen SC, Devina T, Cheng IH, Lien CC, Kao LS, Wang SJ, Fuh JL, Tzeng TT, Huang CY, Shiao YJ, Wong YH. Sci Rep. 2019 Dec 17;9(1):19301. doi: 10.1038/s41598-019-55614-9. PubMed
Rapid generation of regionally specified CNS neurons by sequential patterning and conversion of human induced pluripotent stem cells. Chen M, Maimaitili M, Habekost M, Gill KP, Mermet-Joret N, Nabavi S, Febbraro F, Denham M. Stem Cell Res. 2020 Oct;48:101945. doi: 10.1016/j.scr.2020.101945. Epub 2020 Aug 3. PubMed
Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA. Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460. PubMed
Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia. Ryan SK, Jordan-Sciutto KL, Anderson SA. STAR Protoc. 2020 Dec 1;1(3):100190. doi: 10.1016/j.xpro.2020.100190. eCollection 2020 Dec 18. PubMed
Dyshomeostatic modulation of Ca(2+)-activated K(+) channels in a human neuronal model of KCNQ2 encephalopathy. Simkin D, Marshall KA, Vanoye CG, Desai RR, Bustos BI, Piyevsky BN, Ortega JA, Forrest M, Robertson GL, Penzes P, Laux LC, Lubbe SJ, Millichap JJ, George AL Jr, Kiskinis E. Elife. 2021 Feb 5;10. pii: 64434. doi: 10.7554/eLife.64434. PubMed

Associated Plasmids
NGN2 mmRNA-Based Transcriptional Programming in Microfluidic Guides hiPSCs Toward Neural Fate With Multiple Identities. Tolomeo AM, Laterza C, Grespan E, Michielin F, Canals I, Kokaia Z, Muraca M, Gagliano O, Elvassore N. Front Cell Neurosci. 2021 Feb 12;15:602888. doi: 10.3389/fncel.2021.602888. eCollection 2021. PubMed
Neuron and astrocyte aggregation and sorting in three-dimensional neuronal constructs. Hasan MF, Berdichevsky Y. Commun Biol. 2021 May 17;4(1):587. doi: 10.1038/s42003-021-02104-2. PubMed
Human cell transformation by combined lineage conversion and oncogene expression. Sahu B, Pihlajamaa P, Zhang K, Palin K, Ahonen S, Cervera A, Ristimaki A, Aaltonen LA, Hautaniemi S, Taipale J. Oncogene. 2021 Sep;40(36):5533-5547. doi: 10.1038/s41388-021-01940-0. Epub 2021 Jul 23. PubMed
Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs). Hu R, Zhu X, Yang N. Methods Mol Biol. 2021;2352:117-126. doi: 10.1007/978-1-0716-1601-7_8. PubMed
Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons. Quist E, Ahlenius H, Canals I. Methods Mol Biol. 2021;2352:133-148. doi: 10.1007/978-1-0716-1601-7_10. PubMed
Stem cell-derived neurons reflect features of protein networks, neuropathology, and cognitive outcome of their aged human donors. Lagomarsino VN, Pearse RV 2nd, Liu L, Hsieh YC, Fernandez MA, Vinton EA, Paull D, Felsky D, Tasaki S, Gaiteri C, Vardarajan B, Lee H, Muratore CR, Benoit CR, Chou V, Fancher SB, He A, Merchant JP, Duong DM, Martinez H, Zhou M, Bah F, Vicent MA, Stricker JMS, Xu J, Dammer EB, Levey AI, Chibnik LB, Menon V, Seyfried NT, De Jager PL, Noggle S, Selkoe DJ, Bennett DA, Young-Pearse TL. Neuron. 2021 Nov 3;109(21):3402-3420.e9. doi: 10.1016/j.neuron.2021.08.003. Epub 2021 Sep 1. PubMed
VLDLR and ApoER2 are receptors for multiple alphaviruses. Clark LE, Clark SA, Lin C, Liu J, Coscia A, Nabel KG, Yang P, Neel DV, Lee H, Brusic V, Stryapunina I, Plante KS, Ahmed AA, Catteruccia F, Young-Pearse TL, Chiu IM, Llopis PM, Weaver SC, Abraham J. Nature. 2022 Feb;602(7897):475-480. doi: 10.1038/s41586-021-04326-0. Epub 2021 Dec 20. PubMed
APP and DYRK1A regulate axonal and synaptic vesicle protein networks and mediate Alzheimer's pathology in trisomy 21 neurons. Wu CI, Vinton EA, Pearse RV 2nd, Heo K, Aylward AJ, Hsieh YC, Bi Y, Adeleye S, Fancher S, Duong DM, Seyfried NT, Schwarz TL, Young-Pearse TL. Mol Psychiatry. 2022 Apr;27(4):1970-1989. doi: 10.1038/s41380-022-01454-5. Epub 2022 Feb 23. PubMed
Myc Supports Self-Renewal of Basal Cells in the Esophageal Epithelium. Hishida T, Vazquez-Ferrer E, Hishida-Nozaki Y, Takemoto Y, Hatanaka F, Yoshida K, Prieto J, Sahu SK, Takahashi Y, Reddy P, O'Keefe DD, Rodriguez Esteban C, Knoepfler PS, Nunez Delicado E, Castells A, Campistol JM, Kato R, Nakagawa H, Izpisua Belmonte JC. Front Cell Dev Biol. 2022 Mar 4;10:786031. doi: 10.3389/fcell.2022.786031. eCollection 2022. PubMed
Mapping cis-regulatory elements in human neurons links psychiatric disease heritability and activity-regulated transcriptional programs. Sanchez-Priego C, Hu R, Boshans LL, Lalli M, Janas JA, Williams SE, Dong Z, Yang N. Cell Rep. 2022 May 31;39(9):110877. doi: 10.1016/j.celrep.2022.110877. PubMed
YAP inhibits ERalpha and ER(+) breast cancer growth by disrupting a TEAD-ERalpha signaling axis. Li X, Zhuo S, Zhuang T, Cho YS, Wu G, Liu Y, Mu K, Zhang K, Su P, Yang Y, Zhang CC, Zhu J, Jiang J. Nat Commun. 2022 Jun 2;13(1):3075. doi: 10.1038/s41467-022-30831-5. PubMed
Lipase regulation of cellular fatty acid homeostasis as a Parkinson's disease therapeutic strategy. Fanning S, Cirka H, Thies JL, Jeong J, Niemi SM, Yoon J, Ho GPH, Pacheco JA, Dettmer U, Liu L, Clish CB, Hodgetts KJ, Hutchinson JN, Muratore CR, Caldwell GA, Caldwell KA, Selkoe D. NPJ Parkinsons Dis. 2022 Jun 9;8(1):74. doi: 10.1038/s41531-022-00335-6. PubMed
Orgo-Seq integrates single-cell and bulk transcriptomic data to identify cell type specific-driver genes associated with autism spectrum disorder. Lim ET, Chan Y, Dawes P, Guo X, Erdin S, Tai DJC, Liu S, Reichert JM, Burns MJ, Chan YK, Chiang JJ, Meyer K, Zhang X, Walsh CA, Yankner BA, Raychaudhuri S, Hirschhorn JN, Gusella JF, Talkowski ME, Church GM. Nat Commun. 2022 Jun 10;13(1):3243. doi: 10.1038/s41467-022-30968-3. PubMed
Transition to a mesenchymal state in neuroblastoma confers resistance to anti-GD2 antibody via reduced expression of ST8SIA1. Mabe NW, Huang M, Dalton GN, Alexe G, Schaefer DA, Geraghty AC, Robichaud AL, Conway AS, Khalid D, Mader MM, Belk JA, Ross KN, Sheffer M, Linde MH, Ly N, Yao W, Rotiroti MC, Smith BAH, Wernig M, Bertozzi CR, Monje M, Mitsiades CS, Majeti R, Satpathy AT, Stegmaier K, Majzner RG. Nat Cancer. 2022 Aug;3(8):976-993. doi: 10.1038/s43018-022-00405-x. Epub 2022 Jul 11. PubMed
Production of human entorhinal stellate cell-like cells by forward programming shows an important role of Foxp1 in reprogramming. Bergmann T, Liu Y, Skov J, Mogus L, Lee J, Pfisterer U, Handfield LF, Asenjo-Martinez A, Lisa-Vargas I, Seemann SE, Lee JTH, Patikas N, Kornum BR, Denham M, Hyttel P, Witter MP, Gorodkin J, Pers TH, Hemberg M, Khodosevich K, Hall VJ. Front Cell Dev Biol. 2022 Aug 15;10:976549. doi: 10.3389/fcell.2022.976549. eCollection 2022. PubMed
Molecular convergence between Down syndrome and fragile X syndrome identified using human pluripotent stem cell models. Susco SG, Ghosh S, Mazzucato P, Angelini G, Beccard A, Barrera V, Berryer MH, Messana A, Lam D, Hazelbaker DZ, Barrett LE. Cell Rep. 2022 Sep 6;40(10):111312. doi: 10.1016/j.celrep.2022.111312. PubMed
CASK loss of function differentially regulates neuronal maturation and synaptic function in human induced cortical excitatory neurons. McSweeney D, Gabriel R, Jin K, Pang ZP, Aronow B, Pak C. iScience. 2022 Sep 23;25(10):105187. doi: 10.1016/j.isci.2022.105187. eCollection 2022 Oct 21. PubMed
YAP antagonizes TEAD-mediated AR signaling and prostate cancer growth. Li X, Zhuo S, Cho YS, Liu Y, Yang Y, Zhu J, Jiang J. EMBO J. 2023 Feb 15;42(4):e112184. doi: 10.15252/embj.2022112184. Epub 2023 Jan 2. PubMed
Neuronal-Activity Dependent Mechanisms of Small Cell Lung Cancer Progression. Savchuk S, Gentry K, Wang W, Carleton E, Yalcin B, Liu Y, Pavarino EC, LaBelle J, Toland AM, Woo PJ, Qu F, Filbin MG, Krasnow MA, Sabatini BL, Sage J, Monje M, Venkatesh HS. bioRxiv. 2023 Jan 20:2023.01.19.524430. doi: 10.1101/2023.01.19.524430. Preprint. PubMed
Predictive network analysis identifies JMJD6 and other potential key drivers in Alzheimer's disease. Merchant JP, Zhu K, Henrion MYR, Zaidi SSA, Lau B, Moein S, Alamprese ML, Pearse RV 2nd, Bennett DA, Ertekin-Taner N, Young-Pearse TL, Chang R. Commun Biol. 2023 May 15;6(1):503. doi: 10.1038/s42003-023-04791-5. PubMed
Variants in SART3 cause a spliceosomopathy characterised by failure of testis development and neuronal defects. Ayers KL, Eggers S, Rollo BN, Smith KR, Davidson NM, Siddall NA, Zhao L, Bowles J, Weiss K, Zanni G, Burglen L, Ben-Shachar S, Rosensaft J, Raas-Rothschild A, Jorgensen A, Schittenhelm RB, Huang C, Robevska G, van den Bergen J, Casagranda F, Cyza J, Pachernegg S, Wright DK, Bahlo M, Oshlack A, O'Brien TJ, Kwan P, Koopman P, Hime GR, Girard N, Hoffmann C, Shilon Y, Zung A, Bertini E, Milh M, Ben Rhouma B, Belguith N, Bashamboo A, McElreavey K, Banne E, Weintrob N, BenZeev B, Sinclair AH. Nat Commun. 2023 Jun 9;14(1):3403. doi: 10.1038/s41467-023-39040-0. PubMed
High-Content Small Molecule Screen Identifies a Novel Compound That Restores AP-4-Dependent Protein Trafficking in Neuronal Models of AP-4-Associated Hereditary Spastic Paraplegia. Saffari A, Brechmann B, Boeger C, Saber WA, Jumo H, Whye D, Wood D, Wahlster L, Alecu J, Ziegler M, Scheffold M, Winden K, Hubbs J, Buttermore E, Barrett L, Borner G, Davies A, Sahin M, Ebrahimi-Fakhari D. Res Sq. 2023 Jun 12:rs.3.rs-3036166. doi: 10.21203/rs.3.rs-3036166/v1. Preprint. PubMed
Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons. Sheta R, Teixeira M, Idi W, Oueslati A. STAR Protoc. 2023 Jul 28;4(3):102486. doi: 10.1016/j.xpro.2023.102486. PubMed
FOXP1 orchestrates neurogenesis in human cortical basal radial glial cells. Park SHE, Kulkarni A, Konopka G. PLoS Biol. 2023 Aug 4;21(8):e3001852. doi: 10.1371/journal.pbio.3001852. eCollection 2023 Aug. PubMed
Ascl1 and Ngn2 convert mouse embryonic stem cells to neurons via functionally distinct paths. Vainorius G, Novatchkova M, Michlits G, Baar JC, Raupach C, Lee J, Yelagandula R, Wernig M, Elling U. Nat Commun. 2023 Sep 2;14(1):5341. doi: 10.1038/s41467-023-40803-y. PubMed
Small molecule regulators of microRNAs identified by high-throughput screen coupled with high-throughput sequencing. Nguyen LD, Wei Z, Silva MC, Barberan-Soler S, Zhang J, Rabinovsky R, Muratore CR, Stricker JMS, Hortman C, Young-Pearse TL, Haggarty SJ, Krichevsky AM. Nat Commun. 2023 Nov 21;14(1):7575. doi: 10.1038/s41467-023-43293-0. PubMed
Generation of a stably transfected mouse embryonic stem cell line for inducible differentiation to excitatory neurons. Gu J, Rollo B, Berecki G, Petrou S, Kwan P, Sumer H, Cromer B. Exp Cell Res. 2024 Feb 1;435(1):113902. doi: 10.1016/j.yexcr.2023.113902. Epub 2023 Dec 24. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.