Prevention of transgene silencing during human pluripotent stem cell differentiation.
Uenaka T, Napole A, Saha AD, Sun D, Singavarapu A, Calzada E, Chen J, Erlebach L, McQuade A, Ramos DM, Rigamonti A, Salazar L, Samelson AJ, Sedov K, Welsh NJ, Wild K, Wu Q, Arenas E, Bassett AR, Kampmann M, Kronenberg-Versteeg D, Merkle FT, Schule B, Thompson LM, Skarnes WC, Ward ME, Wernig M
Cell Stem Cell. 2026 Feb 13:S1934-5909(26)00030-5. doi: 10.1016/j.stem.2026.01.007.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239046 | TK4-NLS-EGFP | PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation |
| 239047 | TK4-EGFP | PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation |
| 254452 | TK4_SH4-1_targeting | TK4 targeting vector containing left and right homology arms for the SH4-1 locus |
| 254453 | TK4_SH4-2_targeting | TK4 targeting vector containing left and right homology arms for the SH4-2 locus |
| 254454 | TK4_SH7_targeting | TK4 targeting vector containing left and right homology arms for the SH7 locus |
| 254455 | TK4_CLYBL_targeting | TK4 targeting vector containing left and right homology arms for the CLYBL locus |
| 254456 | TK4_Zim3-dCas9-2xMyc-NLS-T2A-mApple_EF1a-Hygro | TK4 PiggyBac plasmid for stable expression of Zim3-dCas9 during human pluripotent stem cell differentiation |