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TK4_Zim3-dCas9-2xMyc-NLS-T2A-mApple_EF1a-Hygro
(Plasmid #254456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254456 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUCM
  • Total vector size (bp) 17488
  • Vector type
    Mammalian Expression ; PiggyBac
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Zim3-dCas9-2xMyc-NLS-T2A-mApple
  • Species
    Synthetic
  • Insert Size (bp)
    5436
  • Promoter CAG
  • Tag / Fusion Protein
    • T2A-mApple (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    hygroR
  • Species
    Synthetic
  • Insert Size (bp)
    1026
  • Promoter EF1a

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TK4_Zim3-dCas9-2xMyc-NLS-T2A-mApple_EF1a-Hygro was a gift from Marius Wernig (Addgene plasmid # 254456 ; http://n2t.net/addgene:254456 ; RRID:Addgene_254456)

  • For your References section:

    Prevention of transgene silencing during human pluripotent stem cell differentiation. Uenaka T, Napole A, Saha AD, Sun D, Singavarapu A, Calzada E, Chen J, Erlebach L, McQuade A, Ramos DM, Rigamonti A, Salazar L, Samelson AJ, Sedov K, Welsh NJ, Wild K, Wu Q, Arenas E, Bassett AR, Kampmann M, Kronenberg-Versteeg D, Merkle FT, Schule B, Thompson LM, Skarnes WC, Ward ME, Wernig M. Cell Stem Cell. 2026 Feb 13:S1934-5909(26)00030-5. doi: 10.1016/j.stem.2026.01.007. 10.1016/j.stem.2026.01.007 PubMed 41690310
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