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pLV-TetO-hNGN2-eGFP-Puro
(Plasmid #79823)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79823 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Tet-O-FUW
  • Backbone size w/o insert (bp) 8387
  • Total vector size (bp) 10687
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hNGN2-P2A-eGFP-T2A-PuroR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • GenBank ID
    NM_024019.3
  • Entrez Gene
    NEUROG2 (a.k.a. Atoh4, Math4A, NGN2, bHLHa8, ngn-2)
  • Promoter TetO

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    It was modifed from a kind gift from Dr. Sudhof. "Rapid Single-Step Induction of Functional Neurons from Human Pluripotent Stem Cells", Zhang et al.. 2013
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-TetO-hNGN2-eGFP-Puro was a gift from Kristen Brennand (Addgene plasmid # 79823 ; http://n2t.net/addgene:79823 ; RRID:Addgene_79823)
  • For your References section:

    Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells. Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, Brennand KJ. Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 10.1016/j.ymeth.2015.11.019. 10.1016/j.ymeth.2015.11.019 PubMed 26626326