Purpose3rd generation lentiviral vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19780||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8400
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namereverse tetracycline transactivator
SpeciesR. norvegicus (rat)
Insert Size (bp)1025
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
From article, concerning viral infections: For a standard infection in a 35 mmdish (aprox 10^5 cells), 10 microL rtTA+5 microL factors (OCT4, SOX2, KLF4, and NANOG)+2 microL cMYC was used in an overnight infection supplemented with 6 microg/microL polybrene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FUdeltaGW-rtTA was a gift from Konrad Hochedlinger (Addgene plasmid # 19780 ; http://n2t.net/addgene:19780 ; RRID:Addgene_19780)
For your References section:A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. Cell Stem Cell. 2008 Sep 11. 3(3):340-5. 10.1016/j.stem.2008.08.003 PubMed 18786420