Purpose(Empty Backbone) tetracyline-inducible lentiviral vector with no insert for use in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85747||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerRudolf Jaenisch
- Backbone size (bp) 9537
Modifications to backbonepuro resistance added
- Promoter CMV
/ Fusion Protein
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Growth instructionsprone to rearrangement
- Cloning method Restriction Enzyme
- 5′ sequencing primer fuwF (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TetO-FUW-pgk-puro was a gift from Emily Dykhuizen (Addgene plasmid # 85747 ; http://n2t.net/addgene:85747 ; RRID:Addgene_85747)
For your References section:PBRM1 Regulates the Expression of Genes Involved in Metabolism and Cell Adhesion in Renal Clear Cell Carcinoma. Chowdhury B, Porter EG, Stewart JC, Ferreira CR, Schipma MJ, Dykhuizen EC. PLoS One. 2016 Apr 21;11(4):e0153718. doi: 10.1371/journal.pone.0153718. eCollection 2016. 10.1371/journal.pone.0153718 PubMed 27100670