-
Purpose(Empty Backbone) doxycycline-inducible lentiviral vector
-
Depositing Lab
-
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backboneFUW
- Backbone size (bp) 8482
-
Modifications to backboneFUW-tetO-hOCT4 (Addgene #20726) was modified by replacing hOct4 with a new multiple cloning site (MCS)
-
Vector typeMammalian Expression, Lentiviral
- Promoter TRE-CMV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Growth instructionsStbl3, 37ºC
-
Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX 5'-AGCTCGTTTAGTGAACCGTCAGATC-3'
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FUW-tetO-MCS was a gift from Stefano Piccolo (Addgene plasmid # 84008 ; http://n2t.net/addgene:84008 ; RRID:Addgene_84008) -
For your References section:
Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S. Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 10.1016/j.stem.2016.08.009. 10.1016/j.stem.2016.08.009 PubMed 27641305