Purpose(Empty Backbone) doxycycline-inducible lentiviral vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84008||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||84008-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8482
Modifications to backboneFUW-tetO-hOCT4 (Addgene #20726) was modified by replacing hOct4 with a new multiple cloning site (MCS)
Vector typeMammalian Expression, Lentiviral
- Promoter TRE-CMV
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsStbl3, 37ºC
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX 5'-AGCTCGTTTAGTGAACCGTCAGATC-3' (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 84008-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FUW-tetO-MCS was a gift from Stefano Piccolo (Addgene plasmid # 84008 ; http://n2t.net/addgene:84008 ; RRID:Addgene_84008)
For your References section:Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S. Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 10.1016/j.stem.2016.08.009. 10.1016/j.stem.2016.08.009 PubMed 27641305