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FUW-tetO-EGFP
(Plasmid #84041)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84041 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUW
  • Backbone size w/o insert (bp) 8482
  • Total vector size (bp) 10463
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3, 37ºC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
  • Alt name
    EGFP-IRES-Puro
  • Insert Size (bp)
    1947
  • Promoter TRE-CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer pBluescriptKS
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The construct FUW-tetO-EGFP was obtained from FUW-tetO-hOct4 (Addgene #20726) by replacing hOct4 with the EGFP-ires-puro cassette from PL-SIN-EOS-C(3+)-EiP (Addgene #21313).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW-tetO-EGFP was a gift from Stefano Piccolo (Addgene plasmid # 84041 ; http://n2t.net/addgene:84041 ; RRID:Addgene_84041)
  • For your References section:

    Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ. Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S. Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 10.1016/j.stem.2016.08.009. 10.1016/j.stem.2016.08.009 PubMed 27641305