FUW-tetO-EGFP
(Plasmid #84041)

• Purpose: 3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
• Depositing Lab: Stefano Piccolo
• Publication: Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 10.1016/j.stem.2016.08.009.

Backbone

• Vector backbone: FUW
• Backbone size w/o insert (bp): 8482
• Total vector size (bp): 10463
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3, 37ºC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced Green Fluorescence Protein, Puromycin resistance gene
• Alt name: EGFP-IRES-Puro
• Insert Size (bp): 1947
• Promoter: TRE-CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: pBluescriptKS

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The construct FUW-tetO-EGFP was obtained from FUW-tetO-hOct4 (Addgene #20726) by replacing hOct4 with the EGFP-ires-puro cassette from PL-SIN-EOS-C(3+)-EiP (Addgene #21313).