pLenti CMV rtTA3 Hygro (w785-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26730||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 8669
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsStbl3, 37oC
Copy numberHigh Copy
Gene/Insert nameTetracycline repressor A3 mutant
Insert Size (bp)707
- Cloning method Restriction Enzyme
- 5′ cloning site Xba I (not destroyed)
- 3′ cloning site Pvu II (destroyed during cloning)
- 5′ sequencing primer CMVforw
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe rtTA3 element was a gift from Dr. Dominic Esposito (SAIC Frederick)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
This plasmid can be used to generate Tet-On advanced cell lines. It has been reported that it works
better than other tetracycline repressor (Markusic et al. 2005).
This plasmid has a mismatch in the first attB site. The consequences of this mismatch are not clear, however depositor successfully made 2 different cell lines with that construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMV rtTA3 Hygro (w785-1) was a gift from Eric Campeau (Addgene plasmid # 26730 ; http://n2t.net/addgene:26730 ; RRID:Addgene_26730)