pLenti CMVtight Puro DEST (w768-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26430||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 9402
Vector typeMammalian Expression, Lentiviral ; Tet-On Advanced Destination vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsDB3.1 or ccDB survival, 37oC.
Copy numberLow Copy
Gene/Insert nameno insert
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVtightFORW
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe CMVtight element was a gift from Dr. Dominic Esposito.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMVtight Puro DEST (w768-1) was a gift from Eric Campeau (Addgene plasmid # 26430 ; http://n2t.net/addgene:26430 ; RRID:Addgene_26430)