pLenti CMVtight Blast DEST (w762-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26434||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 9116
Vector typeMammalian Expression, Lentiviral ; Tet-On Advanced Destination vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsDB3.1 or ccDB survival, 37oC
Copy numberHigh Copy
Gene/Insert nameno insert
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVtightFORW
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe CMVtight element was a gift from Dr. Dominic Esposito.
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Need to be transduced into a Tet-On Advanced cell line for expression of the GOI.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMVtight Blast DEST (w762-1) was a gift from Eric Campeau (Addgene plasmid # 26434 ; http://n2t.net/addgene:26434 ; RRID:Addgene_26434)