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pCGN N-Ras wt
(Plasmid #14723)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14723 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCGN
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-ras
  • Species
    H. sapiens (human)
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, N-ras, NCMS, NRAS1, NS6)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Channing Der
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGN N-Ras wt was a gift from Adrienne Cox (Addgene plasmid # 14723 ; http://n2t.net/addgene:14723 ; RRID:Addgene_14723)
  • For your References section:

    Mammalian expression vectors for Ras family proteins: generation and use of expression constructs to analyze Ras family function. Fiordalisi JJ, Johnson RL, Ulku AS, Der CJ, Cox AD. Methods Enzymol. 2001 . 332():3-36. PubMed 11305105