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pBabe N-Ras 61K
(Plasmid #12543)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12543 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-Puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRas 61K
  • Alt name
    NRAS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    570
  • Mutation
    61K constitutively activated.
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, N-ras, NCMS, NRAS1, NS6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The original N-Ras was cloned as a full-length cDNA (containing 5' and 3' UTRs) Bam-Bam into pCGN, and was therefore transfered via Bam cuts into other vectors, including pBABE, and orientation checked. So, a 1.2 kb band is expected. There should be about 100 bp of 5'UTR, 527 bp N-Ras, and the rest 3'UTR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe N-Ras 61K was a gift from Channing Der (Addgene plasmid # 12543 ; http://n2t.net/addgene:12543 ; RRID:Addgene_12543)
  • For your References section:

    Oncogenic Ras activation of Raf/mitogen-activated protein kinase-independent pathways is sufficient to cause tumorigenic transformation. Khosravi-Far R, White MA, Westwick JK, Solski PA, Chrzanowska-Wodnicka M, Van Aelst L, Wigler MH, Der CJ. Mol Cell Biol. 1996 Jul . 16(7):3923-33. PubMed 8668210