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pBabe-Puro-BRAF-V600E
(Plasmid #15269)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15269 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRAF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    Valine 600 to Glutamate (mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF1, NS7, RAFB1)
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Puro-BRAF-V600E was a gift from William Hahn (Addgene plasmid # 15269 ; http://n2t.net/addgene:15269 ; RRID:Addgene_15269)
  • For your References section:

    Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC. Cell. 2007 Jun 15. 129(6):1065-1079. 10.1016/j.cell.2007.03.052 PubMed 17574021