Braf
(Plasmid #40775)

• Depositing Lab: Dustin Maly
• Publication: various new plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1-Hygro
  (Search Vector Database)
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 7900
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Braf
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• Mutation: Starting Met removed
• Entrez Gene: BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHR

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry