Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917.

Kan A, Gelfat I, Emani S, Praveschotinunt P, Joshi NS
ACS Synth Biol. 2020 Dec 10. doi: 10.1021/acssynbio.0c00466. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
137921	pM1s3AsG	E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression
137922	pM1s3TsR	E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression
137923	pM2s2TsR	E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression
137924	pM2s2AsG	E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
137944	pM2s2AsR_TS-E10	E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
137945	pM1s3AsR_TS-C7	E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
137946	pM1s3ATScsg-Etag	E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression
141293	pCryptDel4.8	Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.