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Orthogonal translation enables heterologous ribosome engineering in E. coli.

Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH
Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
156321pNK110dExpresses heterologous rRNA
156322pRF001d Expresses heterologous rRNA
156323pRF002dExpresses heterologous rRNA
156324pAB172aExpresses heterologous rRNA
156325pNK007pExpresses heterologous rRNA
156326pRF004dExpresses heterologous rRNA
156327pRF005dExpresses heterologous rRNA
156328pRF006dExpresses heterologous rRNA
156329pRF007dExpresses heterologous rRNA
156330pRF008dExpresses heterologous rRNA
156331pNK041pExpresses heterologous rRNA
156332pRF010dExpresses heterologous rRNA
156333pNK059pExpresses heterologous rRNA
156334pNK013pExpresses heterologous rRNA
156335pNK045pExpresses heterologous rRNA
156336pAC027Expresses heterologous rRNA
156337pNK003pExpresses heterologous rRNA
156338pAC018Expresses heterologous rRNA
156339pAC044Expresses heterologous rRNA
156340pAC037Expresses heterologous rRNA
156341pNK053pExpresses heterologous rRNA
156342pAC036Expresses heterologous rRNA
156343pAC030Expresses heterologous rRNA
156344pAC042Expresses heterologous rRNA
156345pAC038Expresses heterologous rRNA
156346pAC040Expresses heterologous rRNA
156347pAC039Expresses heterologous rRNA
156348pAC041Expresses heterologous rRNA
156349pRF028dExpresses heterologous rRNA
156350pAC019Expresses heterologous rRNA
156351pRF030dExpresses heterologous rRNA
156352pRF031dExpresses heterologous rRNA
156353pRF032dExpresses heterologous rRNA
156354pRF033dExpresses heterologous rRNA
156355pRF034dExpresses heterologous rRNA
156356pRF035dExpresses heterologous rRNA
156357pRF036dExpresses heterologous rRNA
156358pAB184aExpresses heterlogous ribosomal proteins
156359pAB184b3Expresses heterlogous ribosomal proteins
156360pAB184c3Expresses heterlogous ribosomal proteins
156361pAB184d3Expresses heterlogous ribosomal proteins
156362pAB184e2Expresses heterlogous ribosomal proteins
156363pAB184f2Expresses heterlogous ribosomal proteins
156364pAB184gExpresses heterlogous ribosomal proteins
156365pAB184hExpresses heterlogous ribosomal proteins
156366pNK083bExpresses heterlogous ribosomal proteins
156367pNK083dExpresses heterlogous ribosomal proteins
156368pNK108hExpresses heterlogous ribosomal proteins
156369pNK131d Expresses heterlogous ribosomal proteins
156370pNK132dExpresses heterlogous ribosomal proteins
156371pNK055bExpresses mCherry
156372S4246Erythromycin-resistant S2060 derivative
156373pAB140j8Erythromycin-dependent sfGFP reporter
156374pNK141a2Expresses orthogonal RBS Sirius reporter
156375pNK141b2Expresses orthogonal RBS mTagBFP reporter
156376pNK141c2Expresses orthogonal RBS mCerulean reporter
156377pNK141d2Expresses orthogonal RBS Sapphire reporter
156378pNK141e2Expresses orthogonal RBS mEmerald reporter
156379pNK141f2Expresses orthogonal RBS Venus reporter
156380pNK141g2Expresses orthogonal RBS mPapaya reporter
156381pNK141h2Expresses orthogonal RBS mScarlet-I reporter
156382pNK141i2Expresses orthogonal RBS mCherry reporter
156383pNK141j2Expresses orthogonal RBS Katushka-9-5 reporter
156384pNK141k2Expresses orthogonal RBS mCarmine reporter
156385pNK141l2Expresses orthogonal RBS LSS-mKate2 reporter
156386pNK141m2Expresses orthogonal RBS MiCy reporter
156387pNK141n2Expresses orthogonal RBS E2-Crimson reporter
156388pNK141o2Expresses orthogonal RBS mMaroon1 reporter

Antibodies from Article