Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy.
Chang Y, Syahirah R, Wang X, Jin G, Torregrosa-Allen S, Elzey BD, Hummel SN, Wang T, Li C, Lian X, Deng Q, Broxmeyer HE, Bao X
Cell Rep. 2022 Jul 19;40(3):111128. doi: 10.1016/j.celrep.2022.111128.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157742 | AAVS1-Puro CAG IL13-CAR | knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression |
| 157743 | AAVS1-Puro CAG CLTX-CAR | knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression |
| 157744 | AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR | knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression |
| 170822 | PiggyBac EF1a-eGFP-Puro U6 PPP2CA g1 | PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown |
| 170823 | PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2 | PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown |
| 170824 | PiggyBac EF1a-eGFP-Puro U6 MMP2 g1 | PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown |
| 170825 | PiggyBac EF1a-eGFP-Puro U6 MMP2 g2 | PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown |
| 170826 | PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1 | PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown |
| 170827 | PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2 | PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown |
| 170828 | PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1 | PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown |
| 170829 | PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2 | PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown |
| 170830 | AAVS1-Puro CAG eGFP NLS | Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus |