Artificial cells drive neural differentiation.
Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet ML, Mansy SS
Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159394 | DT033A | Constitutive murine mature BDNF expression, controlled under E.coli tac promoter |
| 159395 | DT034A | Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter |
| 159396 | DT036A | Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tet promoter |
| 159397 | DT035A | Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter |
| 159398 | DT037A | Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter |
| 159399 | DT038A | Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter |
| 159400 | DT039A | Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter |
| 159401 | DT040A | Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter |
| 159402 | DT041A | Constitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancer |
| 159403 | DT042A | Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter |
| 159404 | DT043A | Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter |
| 159405 | DT044A | Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30) |
| 159407 | DT046A | Constitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter |
| 159408 | DT047A | Constitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter |
| 159409 | DT048A | Constitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter |
| 159410 | DT049A | Constitutive murine mature BDNF expression, controlled under E.coli tet promoter |
| 159411 | DT050A | Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter |