DT034A
(Plasmid #159395)

• Purpose: Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
• Depositing Lab: Sheref Mansy
• Publication: Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep.

Backbone

• Vector backbone: pSB1A3
• Backbone manufacturer: iGEM parts registry
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
• Species: Clostridium perfringens
• Insert Size (bp): 2807
• Promoter: J23117 and pLux promoter
• Tags / Fusion Proteins:
  • FLAG/FLAG (N terminal on insert)
  • pdt#3 from Collins Nat. Biotech. 2014 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: VF2 primer (iGEM parts registry)
• 3′ sequencing primer: VR primer (iGEM parts registry)

Resource Information

• Supplemental Documents:
  • DT034A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry