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Artificial cells drive neural differentiation.
Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet ML, Mansy SS
Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
159394DT033AConstitutive murine mature BDNF expression, controlled under E.coli tac promoter
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159395DT034AConstitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
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159396DT036AConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli tet promoter
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159397DT035AConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter
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159398DT037AConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter
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159399DT038AConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter
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159400DT039AConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter
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159401DT040AConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter
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159402DT041AConstitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancer
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159403DT042AInducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter
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159404DT043AConstitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter
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159405DT044AInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)
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159407DT046AConstitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
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159408DT047AConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
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159409DT048AConstitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
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159410DT049AConstitutive murine mature BDNF expression, controlled under E.coli tet promoter
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159411DT050AConstitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter
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