Addgene: Artificial cells drive neural differentiation.

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Sheref Mansy
Toparlak et al

Artificial cells drive neural differentiation.
Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet ML, Mansy SS
Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
159394	DT033A	Constitutive murine mature BDNF expression, controlled under E.coli tac promoter
159395	DT034A	Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
159396	DT036A	Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tet promoter
159397	DT035A	Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter
159398	DT037A	Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter
159399	DT038A	Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter
159400	DT039A	Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter
159401	DT040A	Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter
159402	DT041A	Constitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancer
159403	DT042A	Inducible superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter
159404	DT043A	Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter
159405	DT044A	Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)
159407	DT046A	Constitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
159408	DT047A	Constitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
159409	DT048A	Constitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
159410	DT049A	Constitutive murine mature BDNF expression, controlled under E.coli tet promoter
159411	DT050A	Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

Antibodies from Article