Bioluminescent Antibodies through Photoconjugation of Protein G-Luciferase Fusion Proteins.
Wouters SFA, Vugs WJP, Arts R, de Leeuw NM, Teeuwen RWH, Merkx M
Bioconjug Chem. 2020 Mar 18;31(3):656-662. doi: 10.1021/acs.bioconjchem.9b00804. Epub 2020 Jan 22.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169105 | pET28a Gx-NL | Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine. |
| 169106 | pET28a Gx-mNG-NL | Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine. |
| 169107 | pET28a Gx-NL2 | Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine. |
| 169108 | pET28a Gx-NL3 | Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine. |