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pET28a Gx-NL
(Plasmid #169105)


Item Catalog # Description Quantity Price (USD)
Plasmid 169105 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6043
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    High Copy


  • Gene/Insert name
    protein G - NanoLuc
  • Species
  • Insert Size (bp)
  • Mutation
    Ala 24 stop
  • Promoter T7
  • Tags / Fusion Proteins
    • strep-tag (N terminal on insert)
    • his-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a Gx-NL was a gift from Maarten Merkx (Addgene plasmid # 169105 ; ; RRID:Addgene_169105)
  • For your References section:

    Bioluminescent Antibodies through Photoconjugation of Protein G-Luciferase Fusion Proteins. Wouters SFA, Vugs WJP, Arts R, de Leeuw NM, Teeuwen RWH, Merkx M. Bioconjug Chem. 2020 Mar 18;31(3):656-662. doi: 10.1021/acs.bioconjchem.9b00804. Epub 2020 Jan 22. 10.1021/acs.bioconjchem.9b00804 PubMed 31909607