Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes.
Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, Swint-Kruse L
Proteins. 2021 Aug 8. doi: 10.1002/prot.26206. PubMed Article
Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, Swint-Kruse L
Proteins. 2021 Aug 8. doi: 10.1002/prot.26206. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170107 | UUUUA-pHG165c | Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain. |
| 170108 | MMMMA-pHG165c | Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain. |
| 170110 | XXXXA-pHG165c | Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain. |
| 170111 | XXXXA_Q182A_R287H-pHG165c | Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain. |
| 170112 | RRRAA-pHG165c | Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains. |
| 170113 | RRRAA-5-mut-pHG165c | Constitutively expressed, mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains. |
| 170114 | AraC-pHG165c WT | Constitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose. |
| 172602 | BW-Para | The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays. |